In The Ultimate Test, Jessie decided to take the Pokémon League Admissions Exam to see if she could enter the Indigo Plateau Conference and add that achievement to her résumé. However, during the theory test, she rose from her seat and started to argue with the instructor, which led to her being expelled. Jessie also loves popularity when she aspires to become a Kalos Queen and became popular with many people. Like many female characters in the manga, Jessie is depicted with an oversized bust, though this is edited down for VIZ Media's English translation.

In Party Dancecapades!, Jessie and her team members decided to attend a dance party hosted by Monsieur Pierre. After seeing Serena and her friends go inside, Meowth noted that it would be a perfect chance to catch Pikachu. Jessie, however, decided to let go of these plans in favor of attending the dance party and decided on James being her date while Gourgeist decided Inkay to be hers. Later, she was seen flailing James during their dance. With the dance party over, Monsieur Pierre announced a Tag Battle and Miette and James were partnered against Ash and Serena. Despite James's chagrin, Jessie sternly told him to win the battle but he lost, leading to her and her Gourgeist eating from the table behind them out of frustration. Jessie then became eligible to participate in the Sinnoh Grand Festival, and she made it into the semi-finals, but lost to Dawn. She also participated in the Pokémon Summer Camp and got a diploma/credit for having studied (she was disguised as Jessilina). After the Sinnoh Grand Festival, Jessie abandoned the Jessilina alias.Both Misty and Jessie were voiced by Rachael Lillis in the 4Kids dub, then they switched to Michele Knotz when TPCi took over. The trio later participates in their boss Giovanni's Operation Tempest in both Meloetta and the Undersea Temple! and Unova's Survival Crisis!. Jessie and James fought both Cilan and Iris using his Yamask and her Woobat against Pansage and Axew. They are amused to see their boss, Giovanni, defeat Ash and his Pikachu using Persian as his battling Pokémon as well as to capture Meloetta in their aircraft. The trio reappeared in An Epic Defense Force!, where they tried to capture a Meloetta for a future job: Operation Jessie is very close with Arbok as she has known it since it was an Ekans. Also, Jessie would do anything to protect Arbok and it would do the same for her. However, Jessie released Arbok to be with its own kind. Despite being apart, Jessie knows her relationship with Arbok will always be with her. While in the anime, the nature of Jessie's relationship with James is not explicitly stated, in the epilogue of the manga, James is shown confessing his love to Jessie. They are shown afterwards married with Jessie visibly pregnant.

She along with her teammates and her Wobbuffet worked as Malva's assistants during the Lumiose Conference from A League of His Own! to Down to the Fiery Finish!, with the aim of earning money and capturing any strong Pokémon in the Kalos League. However, they were not very pleased with their jobs and felt that they were over-working; despite this, they did not quit their jobs. In Kalos League Passion with a Certain Flare!, they made it a point to steal the two finalists, Ash and Alain's, Pokémon after the battle, but could not execute their plan due to Team Flare wreaking havoc in Lumiose City. When Malva decided to abandon the broadcasting and confront Lysandre in A Towering Takeover!, Jessie, as Jessimurrow, took to narrating the calamity herself, until the broadcast was interrupted by Team Flare. In Coming Apart at the Dreams!, she, in disguise of a news reporter, set out with her teammates, Serena, Mairin, and Professor Sycamore towards Lysandre Labs in an attempt to take over the base and bring Team Flare down.In The First Day of the Rest of Your Life!, she and Gourgeist participated in an Exhibition Showcase held in Lumiose City, specifically planned to help the citizens recover from the gloom of the disaster by spreading happiness and excitement. In Mimikyu Unmasked!, Jessie can be a shopaholic and spent all of her team's money at a shopping mall. She also has a talent for sewing and used her skill to fix Mimikyu's costume. Starting in Hoenn, Jessie became a Pokémon Coordinator, entering every Contest that she could. She frequently made it to the battle round, but usually lost there. Although Jessie entered mostly every Contest in Hoenn and Kanto, she lost in all of them, whether it was the first match of the Battle Rounds or the finals.